KMID : 0380020060210050336
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Korean Journal of Biotechnology and Bioengineering 2006 Volume.21 No. 5 p.336 ~ p.340
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Production of Extracellular Polysaccharide by Perfusion Culture of Angelica gigas Nakai Suspension Cells
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Kim Young-Hwa
Kim Ik-Hwan Kim Dong-Il
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Abstract
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High-density perfusion cultivation was performed to produce extracellular polysaccharide (ECP) as immunostimulating agents in suspension cell cultures of Angelica gigas Nakai. In batch culture, the maximum cell density was 16.8 gDCW/L at day 6 and 0.9 g/L of ECP was obtained at day 8. When the medium exchange was started at the fifth day after inoculation for the perfusion culture, high concentration of the cells at 23.8 gDCW/L could be achieved with continuous production of ECP. Treatments of ultrasound and Pluronic F-68 were found to be helpful for the secretion of intracellular ECP into the culture medium.
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KEYWORD
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Angelica gigas, extracellular polysaccharide, perfusion culture, ultrasound, Pluronic F-68
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